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The level of total IgE in human bronchoalveolar lavage fluid (BALF) has been quantified by a new murine anti-human IgE immunoassay, employing a monoclonal antibody as a secondary antibody. The assay has a detection limit of 0.1 microgram of total IgE per ml of BALF and therefore allows lower values to be detected than previous methods. The present study was carried out to evaluate the accuracy of the assay for quantification of total IgE in BALF and to determine if sensitivity could be increased further. Total IgE in BALF was determined from 93 samples obtained from 50 children undergoing diagnostic bronchoscopy for bronchial hyper-reactivity. Thirty-two of these patients had other conditions on the basis of which they were referred for bronchoscopy. In addition four samples of normal BALF were analysed to give a normal range for comparison with BALF obtained from sensitized patients. A significant correlation was found between the values obtained by these two methods (
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