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_PhotoShop Light_ is a version of the popular Photoshop program for mobile devices.
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were discarded, as well as all parasites. The total number of reads per sample was between 1.87×10^7^ and 6.28×10^7^ reads with a median of 1.98×10^7^ reads per sample (Table [2](#Tab2){ref-type=”table”}). Because of the high-sequence-depth reads, it was possible to obtain a low-coverage estimate of the overall genetic diversity of the parasite. For each site and each time point, we first checked for possible chimeric sequences, trimming the sequences and removing reads containing ambiguous bases. Genotyping was performed using the kSNP sorter2 software \[[@CR52]\] on reads with a minimum length of 90 bp (Table [2](#Tab2){ref-type=”table”}). Two datasets were generated: the first one included all kSNP loci, the second one only those loci which gave a clearly interpretable kSNP call (Additional file [1](#MOESM1){ref-type=”media”}: Table S2). Phylogenetic analysis and population genetics analysis {#Sec14} —————————————————— Phylogenetic analyses were performed as previously described \[[@CR17], [@CR53]\]. Briefly, for each strain, nucleotide sequences of microsatellite and kSNP loci were aligned using ClustalW \[[@CR54]\] and the alignment were manually refined. Phylogenetic trees were reconstructed from a concatenated set of all kSNP alleles by Neighbour Joining (NJ) and Maximum-Likelihood (ML) algorithms implemented in the software Geneious version 6.1.7 (Biomatters, Auckland, New Zealand) \[[@CR55]\] and FastTree version 2 \[[@CR56]\]. Gene diversity was estimated for each strain at each locus and overall for each strain from its population (Table [1](#Tab1){ref-type=”table”}). This overall diversity was estimated from the polymorphic information content (PIC) calculated in Cervus 3.0.3 \[[@CR57]\] using the formula: 2 J/ (P~tot~ − J), where P~tot~ is the total number of pairs, J is the number of polymorphic pairs (alleles which differ in only one base
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Minimum: OS: Windows 7/8 Processor: Intel Core i5 or AMD equivalent Memory: 8 GB RAM Graphics: Intel HD 4000 or AMD HD 7000 equivalent DirectX: Version 11 Storage: 250 GB available space Additional Notes: Windows 8.1 users must also have an internet connection in order to utilize some of the game’s features. Recommended: Processor: Intel Core i7 or AMD equivalent Memory: 12 GB RAM
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